In Vivo와 In Vitro상에서 케라틴 함량에 따른 PLGA 지지체의 염증 반응
분야
의약학 > 의공학
저자
홍현혜 ( Hyun Hye Hong ) , 김수진 ( Su Jin Kim ) , 김순희 ( Soon Hee Kim ) , 김혜린 ( Hye Lin Kim ) , 박종학 ( Jong Hak Park ) , 이동원 ( Dong Won Lee ) , 이종문 ( John M Rhee ) , 강길선 ( Gil Son Khang )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2009년, 제6권 제4호, 711~716페이지(총6페이지)
파일형식
2a800394.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    This study was designed to investigate the effect of keratin as a natural material on the local inflammatory reaction of PLGA in vitro and in vivo. We manufactured five different ratios of PLGA/keratin hybrid materials, with each material containing 0, 10, 20, 40 and 80 wt% of keratin of PLGA by solvent casting/salt leaching method. To evaluate the influence of keratin content in PLGA materials on cell viability, NIH/3T3 mouse fibroblasts were cultured in PLGA and the 5 different ratios of PLGA/keratin scaffold. PLGA/keratin films for the in vivo study were implanted in 5-week-old Wister rats for 10 days. Cell proliferation activity was measured using WST assay. ELISA for TNF-a was conducted to evaluate the inflammatory response of cells on the PLGA/keratin scaffolds, the lowest figures showed in PLGA scaffold contained 20% keratin. We have shown that the number of vital cells was higher in the culture of the scaffold containing keratin. Analysis of histological and immunohistochemical in PLGA/keratin hybrid constructs at day 10 after implantation showed that keratin moderate immune response. This result suggest that 20% keratin of PLGA could reduce inflammatory reaction of PLGA.
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