In Vitro상에서 탈미네랄화 골분으로 제조된 용액을 함침시킨 PLGA 지지체의 연골 재생 효과
의약학 > 의공학
홍현혜 ( Hyun Hye Hong ) , 김수진 ( Su Jin Kim ) , 김순희 ( Soon Hee Kim ) , 박종학 ( Jong Hak Park ) , 강길선 ( Gilson Khang )
조직공학과 재생의학 2010년, 제7권 제2호, 8~14페이지(총7페이지)
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    Adult articular cartilage tissue has poor capability of self-repair. For the effective articular cartilage repair and regeneration, numerous studies have been focused on tissue engineering approaches motivated by the clinical need. In this study, we developed synthetic/natural hybrid scaffolds with poly (lactide-co-glycolide)(PLGA) and demineralized bone solution (DB solution) for the application of articular cartilage regeneration. Composite scaffolds of PLGA penetrated DB solution (PLGA-pen-DBP) were manufactured by a solvent casting/salt leaching method soaked in DB solution. The chondrocytes were seeded in the PLGA-pen-DBP scaffolds and their viability was measured by MTT assay. Morphological observation for the scaffolds, biological assay for collagen and sulfated- glycosaminoglycan (sGAG) and PCR were performed. In SEM observation, we observed that PLGA-pen- DBP scaffolds have uniform porosity. Scaffolds containing DB solution were higher cell viability and contents of sGAG and collagen than only PLGA scaffolds. The engineered PLGA-pen-DBP scaffolds have uniform porosity, high cell viability, and relatively high contents of sGAG and collagen as well as chondrocytes contents. This result indicates that may serve as a potential cell delivery vehicle and a structural basis for in vitro tissue engineered articular cartilage.
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