Preparation and Evaluation of PLGA-Silica Scaffold with Human Adipose-Derived Stromal Cells for Bone Tissue Engineering
분야
의약학 > 의공학
저자
( Ki Joo Kim ) , ( Jung Wan Oh ) , ( Guang Zhen Jin ) , ( Jung Ho Lee ) , ( Deuk Young Oh ) , ( Hyun Wook Kang ) , ( Hwan Mook Kim ) , ( Soo Hong Lee ) , ( Dong Woo Cho ) , ( Sang Tae Ahn ) , ( Jong Won Rhie )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2010년, 제7권 제3호, 291~297페이지(총7페이지)
파일형식
2a800543.pdf [무료 PDF 뷰어 다운로드]
  • ※ 본 자료는 참고용 논문으로 수정 및 텍스트 복사가 되지 않습니다.
  • 구매가격
    4,500원
    적립금
    135원 (구매자료 3% 적립)
    이메일 발송  스크랩 하기
    자료 다운로드  네이버 로그인
    영문초록
    One of the main goals of bone tissue engineering is to develop suitable bio-acceptable materials for bone graft substitutes, especially for filling large defects. In the present study, porous poly(lactic-co-glycolic acid) (PLGA)-silica scaffolds were successfully fabricated using indirect microstereolithography technology. The scaffolds were evaluated in vitro by analyzing their microscopic structure, porosity, and stiffness. Following in vitro osteogenic induction, human adipose-derived stromal cells (ADSCs) were seeded in the scaffolds, and the expression levels of osteocalcin mRNA and collagen type I protein were detected by RT-PCR and Western blotting, respectively. We further evaluated the osteoinductive efficacy of the scaffolds in vivo by implanting human ADSC-seeded scaffolds into athymic mice. The silica particles improved the mechanical strength of PLGA scaffolds, and the PLGA-silica scaffolds exhibited better osteogenic potential in vitro than conventional PLGA scaffolds. The in vivo osteogenic events after scaffold implantation were consistent with those observed in vitro. This study demonstrates the potential of PLGA-silica scaffolds seeded with human ADSCs as a useful tool for bone tissue engineering.
    사업자등록번호 220-87-87785 대표.신현웅 주소.서울시 서초구 방배로10길 18, 402호 대표전화.070-8809-9397
    개인정보책임자.박정아 통신판매업신고번호 제2017-서울서초-1765호 이메일 help@reportshop.co.kr
    copyright (c) 2009 happynlife. steel All reserved.