Effects of Mycoplasma Elimination Agent on Maintenance and Cardiogenic Potentials of Human Embryonic Stem Cells

분야: 의약학 > 의공학
저자: ( Yoon Young Kim ) , ( Seung Yup Ku ) , ( Yong Jin Kim ) , ( Myung Soo Cho ) , ( Sun Kyung Oh ) , ( Seok Hyun Kim ) , ( Shin Yong Moon ) , ( Young Min Choi )
발행기관: 한국조직공학·재생의학회
간행물정보: 조직공학과 재생의학 2010년, 제7권 제4호, 419~424페이지(총6페이지)
파일형식: 2a800561.pdf [무료 PDF 뷰어 다운로드]
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영문초록

Human embryonic stem cells (hESCs) are an attractive cell source for cell-based therapy considering their unique characteristics of self-renewal and pluripotency. Optimal culture condition of hESCs is important for the continuous maintenance and differentiation of hESCs. For the elimination or prevention of mycoplasma contamination, antibiotics are used for routine culture of various cell lines, however, their effects on the differentiation of hESCs were not clarified. In this study, we tried to demonstrate the effects of mycoplasma removal agent, plasmocin, on undifferentiated hESCs and derived differentiating cells. There were no significant effects on the morphology and the expression of Oct4, Sox2 and Nanog in undifferentiated hESCs. However, cardiogenic potential of hESCs were significantly affected. The expression of cardiac lineage markers such as Nkx2.5, cTn I and Tbx5 was downregulated in plasmocin group. No dose-response relationship was observed. These results imply that differentiation of hESCs into cardiac lineage can be affected by antibiotics. Therefore, careful selection of infection prevention agent is necessary.

#Human embryonic stem cells # mycoplasma # cardiogenic potential # antibiotics

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