Wnt 억제물질에 의한 지방줄기세포의 연골분화 촉진
의약학 > 의공학
송인선 ( In Sun Song ) , 임군일 ( Gun Il Im )
조직공학과 재생의학 2010년, 제7권 제5호, 610~615페이지(총6페이지)
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    This study was performed to investigate the effect of Wnt inhibitors on chondrogenesis from adipose stem cells (ASCs). Two Wnt antagonists, secreted frizzled-related protein (sFRP)-1 and Dickkopf-related protein (DKK)-1, were treated on passage 3 ASCs in pellet culture. Cell viability was measured at various concentrations (2, 10, 50nM) of each inhibitor by MTT assay, and the inhibition of β-catenin was examined by western blot analysis. Chondrogenic differentiation was analyzed by glycosaminoglycan (GAG) assay and Safranin-O staining, and the gene and protein expression of chondrogenic markers by quantitative RT-PCR and Western blot analysis. The cell viability of ASCs was not significantly affected in the concentration of Wnt inhibitors we used. The expression of β-catenin decreased with the treatment of either inhibitor. There was a significant increase of proteoglycan, and the type II collagen gene and protein expression in ASCs treated with either sFRP-1 or DKK-1. The gene expression of type I collagen decreases while that of type X collagen increased with the treatment of either sFRP-1 or DKK-1. The overall results suggest that the Wnt inhibitiors enhanced chondrogenesis from ASCs.
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