황금작약탕(黃芩芍藥湯)의 RAW 264.7 대식 세포에서의 항염증 효과에 관한 연구
분야
의약학 > 기초한의학
저자
김마룡 ( Ma Ryong Kim ) , 강옥화 ( Ok Hwa Kang ) , 김성배 ( Sung Bae Kim ) , 강희정 ( Hee Jung Kang ) , 김지은 ( Ji Eun Kim ) , 황형칠 ( Hyeong Chil Hwang ) , 김인원 ( In Won Kim ) , 권동렬 ( Dong Yeul Kwon )
발행기관
대한본초학회
간행물정보
대한본초학회지(본초분과학회지) 2012년, 제28권 제1호, 43~50페이지(총8페이지)
파일형식
35301225.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    Objectives : Hwanggeumjakyak-tang (huangqin shaoyao tang, HJT) has been used to treat acute enteritis in traditional oriental medicine. However, there has been a lack of studies regarding the effects of HJT on the inflammatory activities and effector inflammatory disease mechanism about macrophage before is not known. So we examined the effect of HJT water extract on pro-inflammatory mediators in lipopolysaccharide (LPS) - stimulated mouse macrophage, RAW 264.7 cells, Methods : Cells were treated with 2 ug/mL of LPS 1 h prior to the addition of HJT. Cell viability was measured by MTS assay. The production of nitric oxide (NO) was determined by reacting cultured medium with Griess reagent. The expression of cyclooxygenase-2 (COX-2), inducible NO synthase (iNOS) and mitogen-activated protein kinases (MAPKs) was investigated by Western blot, RT-PCR. The content of level of cytokines (prostaglandin (PG) E₂, interleukin (IL)-6, IL-12, Tumor necrosis factor-alpha (TNF-α) and monocyte chemoattractant protein-1 (MCP-1) in media from LPS-stimulated Raw 264.7 cells was analyed by ELISA kit, Results : HJT inhibited the production of NO, PGE₂, IL-6 as well as the expressions of iNOS, COX-2 but did not inhibit the production of IL-12, TNF-α, MCP-1 in the murine macrophage, RAW 264.7 cells. HJT also had suppression effects of LPS- induced MAPKs activation, Conclusion : These results suggest that HJT has an anti-inflammatory therapeutic potential, which may result from inhibition of MAPK phosphorylation, thereby decreasing the expression of pro-inflammatory genes.
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