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분야
농학 > 기타(농학)
저자
황재삼 ( Jae Sam Hwang ) , 박인균 ( In Gyun Park ) , 윤은영 ( Eun Young Yun ) , 정덕웅 ( Duck Oung Jung ) , 윤형주 ( Hyung Joo Yoon ) , 이상범 ( Sang Beom Lee ) , 김성렬 ( Seong Ryul Kim ) , 이준하 ( Joon Ha Lee ) , 김인우 ( In Woo Kim ) , 권용남 ( Young Nam Kwon )
발행기관
한국잠사학회
간행물정보
한국잠사곤충학회지(구 한국잠사학회지) 2012년, 제50권 제2호, 189~193페이지(총5페이지)
파일형식
0n601764.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    To find diapause-related genes, we were performed by differential hybridization with three types of [α-32P]dCTP-labeled total cDNA probes synthesized from diapause-prepared, diapause-maintained and diapause-activated stage of Bombus ignitus queen. Nine individual cDNA clones were found to be differentially expressed in diapause-maintained and diapause- activated stage. Among these clones, BIDC9(BIDC ; Bombus ignitus differentially expressed clone) was analyzed through full-length sequencing and expression pattern analysis. This clone was specifically expressed in the thorax organ. The effect of Juvenile hormone analog(JHA) and CO2 treatment was examined. JHA treatment induced the expression of BIDC9 cDNA clone abruptly after 4 day of treatment. CO2 treatment induced also the clone after 2 day of treatment. BIDC9 cDNA was identified as Bombus ignitus diapause gene contained an open reading frame of 1376 bp encoding 255 amino acids.
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