Soil microarthropods play crucial role in decomposition of organic matters and nutrient cycling in soil ecosystem, and contribute to the energy flow. Herbicide is one of the disturbance factor in soil ecosystem specially in agricultural soil. Non-target effect of the herbicide, Pendimethalin, application on soil microarthropods were studied. Pendimethalin application showed mixed effects on the abundance of soil microarthropods. Short-term reduction of the density was shown in managed agricultural soil system where the natural vegetation density was low. However, in heavily vegetated plots in Pyeongchang, there was no short-term change after treatment. In the latter case, soil microarthropod density was lower from the samples of one year after the treatment than in control plot. Collemboans and predatory mesostigmatid mites were abundant in the study system.