Physiological and Molecular Characterization of a Newly Identified Entomopathogenic Bacteria, Photorhabdus temperata M1021
분야
자연과학 > 생물
저자
( Eun Kyung Jang ) , ( Ihsan Ullah ) , ( Jong Hui Lim ) , ( In Jung Lee ) , ( Jong Guk Kim ) , ( Jae Ho Shin )
발행기관
한국미생물생명공학회(구 한국산업미생물학회)
간행물정보
Journal of Microbiology and Biotechnology 2012년, 제22권 제12호, 1605~1612페이지(총8페이지)
파일형식
05212093.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    The present study concerned the identification and characterization of a novel bacterial strain isolated from entomopathogenic nematodes collected from different regions in Korea. The bacterial isolate M1021 was Gramnegative, bioluminescent, and produced red colonies on MacConkey agar medium. A rod-shaped structure was confirmed by the electron micrograph. Fatty acid composition was analyzed by using the Sherlock MIDI system. The identification was further supported by 16S rDNA sequence analysis, which revealed 96-99% sequence homology with strains of Photorhabdus temperata. The location of the isolated strain of P. temperata in the phylogenetic tree was confirmed and it was named P. temperata M1021. P. temperata M1021 exhibited catalase, protease, and lipase activities when grown on appropriate media supplemented with respective substrates. The culture of P. temperata M1021 exhibited insecticidal activity against the larvae of Galleria mellonella and the activity was the highest after 3-4 days of cultivation with agitating at 28oC under 220 rpm. Antibacterial activity was also observed against Salmonella Typhimurium KCTC 1926 and Micrococcus luteus KACC 10488.
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