Porphyrin Derivatives from a Recombinant Escherichia coli Grown on Chemically Defined Medium
분야
자연과학 > 생물
저자
( Min Ju Lee ) , ( Jin Chun Se ) , ( Hye Jung Kim ) , ( An Sung Kwon ) , ( Soo Youn Jun ) , ( Sang Hyeon Kang ) , ( Pil Kim )
발행기관
한국미생물생명공학회(구 한국산업미생물학회)
간행물정보
Journal of Microbiology and Biotechnology 2012년, 제22권 제12호, 1653~1658페이지(총6페이지)
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05212099.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    We have reported previously that a recombinant Escherichia coli co-expresses aminolevulinic acid (ALA) synthase, an NADP-dependent malic enzyme, and a dicarboxylate transporter-produced heme, an iron-chelated porphyrin, in a succinate-containing complex medium. To develop an industrially plausible process, a chemically defined medium was formulated based on M9 minimal medium. Heme synthesis was enhanced by adding sodium bicarbonate, which strengthened the C4 metabolism required for the precursor metabolite, although a pH change discouraged cell growth. Increasing the medium pH buffering capacity (100mM phosphate buffer) and adding sodium bicarbonate enabled the recombinant E. coli to produce heme at rates 60% greater than those in M9 minimal medium. Adding growth factors (1 mg/l thiamin, 0.01 mg/l biotin, 5 mg/l nicotinic acid, 1 mg/l pantothenic acid, and 1.4 mg/l cobalamin) also induced positive heme production effects at levels twice of heme production in M9-based medium. Porphyrin derivatives and heme were found in the chemically defined medium, and their presence was confirmed by liquid chromatography/mass spectroscopy (LC/MS). The formulated medium allowed for the production of 0.6 ?M heme, 29 ?M ALA, 0.07 ?M coproporphyrin I, 0.21 ?M coproporphyrin III, and 0.23 ?M uroporphyrin in a 3 L pH-controlled culture.
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