In this study, antimicrobial and antioxidative activities were investigated using the hot water extracts of Rhus verniciflua Stokes (RVS) in order to develop RVS as a natural antimicrobial and antioxidative material. The antimicrobial activity was not found against five strains of yeasts (Saccharomyces cerevisiae, Saccharomyces pastorianus, Torulaspora delbrueckii, Zygosaccharomyces rouxii, and Zygosaccharomyces bailii) while the activity was confirmed against five strains of bacteria (Bacillus subtilis, Staphylococcus aureus, Bacillus licheniformis, Escherichia coli, and Pseudomonas aeruginosa) at the RVS extract concentration of 0.2-1.0% (w/v) by plate cultural method. In the investigation for 24 hours by turbidometry, the antimicrobial activity showed a relatively high growth inhibition of 79.7% against Bacillus subtilis. The highest growth inhibition of 82.6% was against Bacillus licheniformis at the RVS extract concentration of 2,000 μg/mL. The lowest growth inhibition was 25.7-44.1% against E. coli at the extract concentration of 250 ?g/mL and 2,000 μg/mL. The total polyphenol and flavonoid contents of the RVS extract were 42.48 mg/g and 5.42 mg/g, respectively. The highest antioxidative activity at 79.15% was observed at the RVS extract concentration of 0.5 mg/mL by DPPH. Also the antioxidative activity by ABTS demonstrated the highest level at 81.56% at the same concentration. It can be gleaned from these results that the hot water extract of RVS has a great potential to be a natural antioxidant and antimicrobial material against gram positive bacteria (Bacillus subtilis and Bacillus licheniformis).