The purpose of this study is to evaluate the protective effect of Lemon balm (Mellisa officinalis) extract on the cytotoxicity induced by cisplatin, pharmaceutic alopecia inducer. To achieve this, cisplatin-induced cytotoxicity was assessed by XTT assay after human skin fibroblasts (Detroit 551) were cultured in media containing various concentrations of cisplatin. And also, the effect of vitamin E was examined on the cisplatin-induced cytotoxicity. For the protective effect of Lemon balm extract on cisplatin-induced cytotoxicity, Detroit 551 cells were pretreated with 80 or 100 μg/mL of Lemon balm extract for 2 hours, and also, antioxidative effects of Lemon balm extract such as 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging activity and lactate dehydrogenase (LDH) activity were analysed. The results were as follows. Cisplatin showed a significant decrease of cell viability in dose dependently, and the XTT50 value was calculated at 25.7 μM. In the effect of vitamin E, it significantly increased cell viability which were decreased by the cisplatin-induced cytotoxicity. In the protective effect of Lemon balm extract on cisplatin-induced cytotoxicity, it significantly increased cell viability which was decreased by cisplatin-induced cytotoxicity, and also it showed the DPPH-radical scavenging activity and LDH inhibitory activity. Therefore, these results suggested that the cytotoxicity of cisplatin may be involved in oxidative stress, and also, Lemon balm extract effectively prevented the cytotoxicity induced by cisplatin via antioxidative effect. Conclusively, the natural extract like Lemon balm may be useful for the development as antioxidative agent via the prevention of the cytotoxicity induced by pharmaceutic alopesia inducer correlated with oxidative stress.