The purpose of this study was to examine the relationship of service education to the emotional labor of dental hygienists and influential factors for their job burnout. The subjects in this study were 155 dental hygienists, on whom a survey was conducted. The findings of the study were as follows: The respondents who ever received service education and were satisfied with that education were better at emotional regulation. After job burnout was classified into physical symptoms and psychological symptoms, a multiple regression analysis was carried out to explore what variables would affect the two subfactors. And it`s found that psychological symptoms were positively affected by emotional dissonance. The above-mentioned findings illustrated that service education had something to do with the emotional labor of the dental hygienists, and that the emotional labor of the dental hygienists had an impact on their job burnout.