ORIGINAL ARTICLE : Potential Immunoinflammatory Role of Staphylococcal Enterotoxin A in Atopic Dermatitis; Immunohistopathological Analysis and in vitro Assay
분야
의약학 > 피부과학
저자
( Hee Woo Lee ) , ( Sung Min Kim ) , ( Jung Min Kim ) , ( Byung Min Oh ) , ( Jun Young Kim ) , ( Han Jin Jung ) , ( Hyun Jung Lim ) , ( Byung Soo Kim ) , ( Weon Ju Lee ) , ( Seok Jong Lee ) , ( Do Won Kim )
발행기관
대한피부과학회
간행물정보
Annals of Dermatology 2013년, 제25권 제2호, 173~180페이지(총8페이지)
파일형식
27728770.pdf [무료 PDF 뷰어 다운로드]
  • ※ 본 자료는 참고용 논문으로 수정 및 텍스트 복사가 되지 않습니다.
  • 구매가격
    4,500원
    적립금
    135원 (구매자료 3% 적립)
    이메일 발송  스크랩 하기
    자료 다운로드  네이버 로그인
    영문초록
    The underlying mechanism of atopic dermatitis (AD) exacerbated by Staphylococcus aureus has not been established. However, we demonstrated recently that the majority of S. aureus strains colonized in the skin of Korean AD patients carried genes encoding staphylococcal enterotoxin A (SEA) and/or toxic shock syndrome toxin-1 (TSST-1). Objective: To clarify the role of staphylococcal superantigen, SEA in AD. Methods: With the lesional skin of 9 AD patients and normal looking skin of one healthy adult, we examined first the expression of SEA, staphylococcal enterotoxin B (SEB), and TSST-1 using immunohistochemical analysis. In addition, we investigated the effects of SEA on the expression of inflammation-related adhesion molecules and cytokines in human HaCaT keratinocytes and Human Umbilical Vein Endothelial Cells (HUVECs) by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and enzyme-linked immunosorbent assay. Results: Staphylococcal protein A (SPA) and SEA were detected with increased immunoreactivity in AD patients. However, TSST-1 showed mild-to-moderate immunoreactivity in AD patients, whereas SEB was minimally detected. In the double immunofluorescence investigation, SEA and SPA were well co-localized. SEA induced upregulation of adhesion moleculesand elicited inflammatory responses in HaCaT keratinocytes and HUVECs. Conclusion: This study demonstrates the importance of SEA as an immunoinflammatory triggering factor of AD in Koreans.
    사업자등록번호 220-87-87785 대표.신현웅 주소.서울시 서초구 방배로10길 18, 402호 대표전화.070-8809-9397
    개인정보책임자.박정아 통신판매업신고번호 제2017-서울서초-1765호 이메일 help@reportshop.co.kr
    copyright (c) 2009 happynlife. steel All reserved.