Purpose: The purpose of this study was to investigate the relationship of the clinical practice stress, fatigue and Yangsaeng in nursing student. Yangsaeng is a traditional health care regimen for the promotion of health and prevention of illness by means of specific principles and methods, which purpose was to improve longevity and healthy life. Method: The subjects of this study were 180 of nursing student. Data were collected by using a self-reported questionnaire and analyzed through descriptive statistics, Pearson`s correlation, t-test, ANOVA and Cronbach`s α. Result: Significant differences in clinical practice stress, fatigue and Yangsaeng according to grade. Clinical practice stress and fatigue was negatively related to Yansaeng. Conclusion: The results of this study suggest that Yangsaeng as oriental health care regimen could lower level of fatigue and clinical practice stress. Therefore, Yangsaeng can be utilized as a nursing intervention of college student of nursing.