영문초록
Background/Aims: We aimed to develop a quantitative en¬zyme-linked immunosorbent assay (ELISA) using whole-cell lysates of Helicobacter pylori 51 and to investigate its validity. Methods: Data from 300 plates were obtained by two differ¬ent operators. Standard sera were used to make a standard curve to analyze the quantity of anti-H. pylori immunoglobulin G (IgG) and IgA antibody. We obtained reproducible data with fewer dilutions of samples by the addition of serially diluted standard serum to each ELISA plate. To evaluate the validity of this ELISA, the 114 H. pylori-positive and -negative sub¬jects were stratified into four age groups, i.e., 0 to 4, 5 to 9, 10 to 15, and 20 to 29 years, before testing. Results: The mean IgG-antibody titers in H. pylori-positive and -negative subjects were 1,766.4 IU/mL and 654.3 IU/mL (p<0.001). The mean IgA-antibody titers in H. pylori-positive and -nega¬tive subjects were 350.1 IU/mL and 193.5 IU/mL (p<0.001). Anti-H. pylori IgG and IgA titers in the four age groups were higher in H. pylori-positive subjects than in H. pylori-negative subjects (p<0.05). Conclusions: Using the current ELISA based on whole-cell lysates of H. pylori 51, reliable anti-H. py¬lori antibody titers were obtained regardless of the subject`s age.
추천자료
[생산기술][재생산기술][항체생산][단백질생산]생산기술과 재생산기술, 생산기술과 항체생산, 생산기술과 단백질생산, 생산기술과 박테리오신생산, 생산기술과 쌀생산, 생산기술과 생명공학, 생산기술 자동화시스템
[PBL] 전신부종과 호흡곤란을 호소한 젊은 여자
[의학, 간호학] 헬리코박터 파일로이 (Helicobacter pylori)
[의학] 박테리아 Bacterial disease
A++ 진단검사(임상병리검사) 임상적의의
신장이식 kidney transplantation
[생명공학재생산기술, lgY생산기술, 의료용단백질생산기술, 박테리오신생산기술] 생명공학의 재생산기술, lgY의 생산기술, 의료용 단백질의 생산기술, 박테리오신 생산발효기술에 관한 분석
의대 본과 4학년 PK과제 호산구성 폐질환
가정간호총정리
[정보탐색]위암(gastric cancer)을 줄이기 위한 Helicobacter pylor의 제거시술(eradication)효과의 평가