ORiginal Article : Development of an ELISA for Quantitative Detection of Immunoglobulin G (IgG) and IgA Antibodies to Helicobacter pylori for Use in Korean Patients with H. pylori-Associated Diseases
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( Ji Hyun Seo ) , ( Jin Su Jun ) , ( Hee Shang Youn ) , ( Jung Sook Yeom ) , ( Ji Sook Park ) , ( Chan Hoo Park ) , ( Hyang Ok Woo ) , ( Woo Kon Lee ) , ( Myung Je Cho ) , ( Kwang Ho Rhee )
Gut and Liver 2013년, 제7권 제4호, 437~442페이지(총6페이지)
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    Background/Aims: We aimed to develop a quantitative en¬zyme-linked immunosorbent assay (ELISA) using whole-cell lysates of Helicobacter pylori 51 and to investigate its validity. Methods: Data from 300 plates were obtained by two differ¬ent operators. Standard sera were used to make a standard curve to analyze the quantity of anti-H. pylori immunoglobulin G (IgG) and IgA antibody. We obtained reproducible data with fewer dilutions of samples by the addition of serially diluted standard serum to each ELISA plate. To evaluate the validity of this ELISA, the 114 H. pylori-positive and -negative sub¬jects were stratified into four age groups, i.e., 0 to 4, 5 to 9, 10 to 15, and 20 to 29 years, before testing. Results: The mean IgG-antibody titers in H. pylori-positive and -negative subjects were 1,766.4 IU/mL and 654.3 IU/mL (p<0.001). The mean IgA-antibody titers in H. pylori-positive and -nega¬tive subjects were 350.1 IU/mL and 193.5 IU/mL (p<0.001). Anti-H. pylori IgG and IgA titers in the four age groups were higher in H. pylori-positive subjects than in H. pylori-negative subjects (p<0.05). Conclusions: Using the current ELISA based on whole-cell lysates of H. pylori 51, reliable anti-H. py¬lori antibody titers were obtained regardless of the subject`s age.
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