Antitumor Effects of Adenovirus-RUNX3 on Human Lung Cancer Cells
의약학 > 내과학
박미영 , 어은영 , 임효정 , 박종선 , 조영재 , 윤호일 , 이재호 , 배석철 , 이춘택
대한결핵 및 호흡기학회
대한결핵및호흡기학회 추계학술발표초록집 2012년, 제114권 149(총1페이지)
0y909805.pdf [무료 PDF 뷰어 다운로드]
  • ※ 본 자료는 참고용 논문으로 수정 및 텍스트 복사가 되지 않습니다.
  • 구매가격
    30원 (구매자료 3% 적립)
    이메일 발송  스크랩 하기
    자료 다운로드  네이버 로그인
    Introduction: Runt-domain transcription factor (RUNX3) has an important role in gene regulation in development. In several cancers, such as gastric cancer, RUNX3 showed tumor suppressive function. Frequent inactivation of RUNX3 by methylation also was found in lung cancer, especially adenocarcinoma. Interestingly, lung adenocarcinoma is observed in RUNX3 heterozygous mice. Recently, inactivation of RUNX3 draws an attention as a potential marker of tumor stem cell. Materials and Methods: We constructed a recombinant adenovirus expressing RUNX3 (ad-RUNX3) by homologous recombination and an adenovirus expressing RUNX3 dominant negative form (ad-RUNX3 DNF) as a nonfunctioning control adenovirus. We tested the function of these adenoviruses in several lung cancer cell lines. Results: Transduction of ad-RUNX3 in lung cancer cell lines showed the production of RUNX3 protein and the expression of RUNX3 was enhanced by vorinostat, histone deacetylase inhibitor which could increase the transduction and transcription of adenoviral transgene. Transduction of ad-RUNX3 showed moderate antitumor effect in lung cancer cell lines. However, combined treatment of ad-RUNX3 with vorinostat showed strong synergistic antitumor effect with enhanced induction of apoptosis. Conclusion: Restoration of RUNX3 via adenoviral vector demonstrated antitumor effect. Further study on ad-RUNX3 will elucidate the role of RUNX3 in lung tumorigenesis.
    사업자등록번호 220-87-87785 대표.신현웅 주소.서울시 서초구 방배로10길 18, 402호 대표전화.070-8809-9397
    개인정보책임자.박정아 통신판매업신고번호 제2017-서울서초-1765호 이메일
    copyright (c) 2009 happynlife. steel All reserved.