Elevation of S100A9 Protein in Sputum of Neutrophilic Inflammation in Severe Uncontrolled Asthma
의약학 > 내과학
장안수 , ( Tae Hyeong Lee ) , ( Jong Sook Park ) , ( Tae Hoon Kim ) , ( Young Suk Choi ) , ( Sung Woo Park ) , ( Soo Taek Uh ) , ( Choon Sik Park )
대한결핵 및 호흡기학회
대한결핵및호흡기학회 추계학술발표초록집 2012년, 제114권 191(총1페이지)
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    Background: Neutrophilic airway inflammation is frequently observed in severe uncontrolled asthma as well as in controlled asthma. However, there is no sputum biomarker to differentiate the two conditions. Objective: To identify biomarkers of severe uncontrolled asthma with neutrophilic airway inflammation. Methods: Sputum with a >70% neutrophil content was pooled from 5 severe uncontrolled asthmatics (UA) and from 10 controlled asthmatics (CA). 2-DE was adopted for differential display proteomics, and candidate proteins were identified using MALDI-TOF MS analysis. S100 calcium binding protein A9 (S100A9) was identified by western blot and its level was measured in sputum from asthmatics with varying disease severity, COPD, and normal controls, by ELISA. Results: 14 protein spots exhibited differences in intensity between severe UA and CA. MALDI-TOF/TOF of these spots revealed an increase in HNP- 2, S100A9, α-amylase, NGAL, 4-aminobutyrate transaminase, and cystatin SA in patients with UA compared to patients with CA. There was a decrease in PLUNC, C3 component, immunoglobulin heavy chain variable region, GFAP1, IgM kappa IIIb SON, MLL-AF4 der (11) fusion protein, cytokeratin 8, and recombinant IgG4 heavy chain. S100A9 was detected at a higher level in western blots of neutrophilic sputum from UA, and significantly increased as measured by ELISA, in neutrophilic UA compared to CA, and COPD. Conclusions: S100A9 in sputum may be a biomarker of neutrophilic inflammation in severe uncontrolled asthma.
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