Background: Inhaled corticosteroids (ICSs) have revolutionized the treatment of asthma and are now the mainstay of therapy for patients with chronic asthma. Interleukin (IL)-33 is a recently characterized IL-1 family cytokine and found to be expressed in inflammatory diseases, including severe asthma and inflammatory bowle disease. IL-32 is a proinflammatory cytokine originally described as a transcript termed NK4 found in activated natural killer (NK) cells and T lymphocytes. analysis of IL-32 and IL-33 cytokine may be important for understanding the pathobiology of steroid asthma at the protein levels. Objective We studied to identify biomarkers in steroid non-responder asthma as comparing protein profiles in plasma between steroid non-responders and steroid responers in asthmatics. Methods: We investigated that the IL-1b, IL-32 and IL-33 level of plasma between nomal control (n=10), steroid non responders (n=14) and steroid responders (n=20) in non-smoker asthmatics by ELISA. Results: We found that IL-33 level was lower significantly in the steroid non-responders (n=14) than the steroid responders(n=20) in asthmatics (p=0.01). But the level of IL-1b, IL-32 had no significant differences between the steroid responders(n=20) and the steroid non-responders (n=14) in asthmatics (p＞0.05). Conclusion: This study suggested that the IL-33 level of plasma might be a biomarker of steroid non- respondes in asthmatics.