Prostaglandin E2 Stimulates Hepatocyte Growth Factor Release in Human Fetal Lung Fibroblast Cell Line through EP2 Receptor and cAMP Pathway

분야: 의약학 > 내과학
저자: 박주헌 , 이혜림 , 김영선 , 정윤정 , 정우영 , 이규성 , 신승수 , 황성철 , ( Stephen I Rennard )
발행기관: 대한결핵 및 호흡기학회
간행물정보: 대한결핵및호흡기학회 추계학술발표초록집 2012년, 제114권 208(총1페이지)
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연관 논문

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원저 : 섬유모세포에서 내독소 자극에 따른 Interleukin - 6 생성의 신호전달과정 ( Intracellular signal transduction of interleukin - 6 production by lipopolysaccharide - stimulated fibroblasts )

Poster Session：PS 0497 ; Nephrology : Paricalcitol Pretreatment Attenuates Apoptosis and Infl ammation in Renal Ischemia-Reperfusion Injury via Prostaglandin E2 Receptor Ep4

원저 : 사람 태아 간세포 및 섬유아세포의 장기 일차 배양 및 계대 배양 ( Long - Term Culture and Passage of Human Fetal Hepatocyte and Fibroblast )

원저 ( Original Articles ) ; 섬유아세포의 증식과 Collagen 합성에 대한 Prostaglandin E2 의 조절작용 ( Modulation of Human Fibroblast Proliferation and Collegan production by Prostaglandin E2 : Association of Intracelluar Cyclic AMP and Changes in Prostaglandin E2 Responsivenes

영문초록

Purpose: Prostaglandin E2 (PGE2) is increased in the lower respiratory tract of patients with COPD. Hepatocyte growth factor (HGF) plays a role in modulating epithelial cell proliferation. In some mesenchymal cells, PGE2 modulates HGF production. This study was to see if PGE2 modulates HGF production and to determine the specific receptors involved. Methods: Human fetal lung fibroblasts (HFL-1) were cultured in Dulbecco`s Modified Eagle`s Medium DMEM) with 10% fetal calf serum. For stimulation, media were changed to DMEM without serum and supplemented with PGE2 or specific agonists and antagonists for each of the four E-prostanoid (EP) receptors. Media were harvested and assayed for HGF by ELISA.Forskolin, KT-5720 (PKA antagonist), and wortmannin were treated to investigate the downstream pathway. Results: Release of HGF was stimulated by PGE2 (p＜0.05). The effect was greater as cells became confluent in culture over 10 days. Both PGE2 and EP2 agonist stimulated HGF release in a concentration dependent manner. EP2 antagonist inhibited the stimulatory effect of PGE2 and EP2 agonist. The other EP agonists and EP antagonists did not alter HGF release.Forskolin treatment also enhanced HGF release (p＜0.05). KT-5720 inhibited HGF release (p＜0.05) but wortmannin did not. Conclusion: HGF production in HFL-1 is stimulated by PGE2 acting on EP2 receptor. Forskolin has a similar effect suggesting that the effect is mediated by modulation of cAMP. The pathway of HGF release through the stimulation by PGE2 is a potential target to modulate lung tissue repair.

[약리학] 아스피린의 항암제 효과(영문)

염증 과수복 Inflammationandrepair

[발생공학] 배아 줄기 세포 연구(영문)

증후군의 정의 및 종류, 원인, 증상, 대책, 최근 연구동향을 통한 나의 의견 정리

분자생물학 - 세포간의 상호관계를 유지하기 위해서 필요한 신호전달체계[signal transduction]에 대해서

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