Purpose: A genome-wide association study has identified the 15q25 region as being associated with the risk of chronic obstructive pulmonary disease (COPD) in Caucasians. This study intended as a confirmatory assessment of this association in a Korean population. Methods: The rs6495309C＞T polymorphism in the promoter of nicotinic acetylcholine receptor alpha subunit 3 (CHRNA3) gene was investigated in a case-control study that consisted of 406 patients with COPD and 394 healthy control subjects. Results: The rs6495309 CT or TT genotype was associated with a significantly decreased risk of COPD when compared to the rs6495309 CC genotype (adjusted odds ratio=0.69, 95% confidence interval=0.50-0.95, P=0.02). The effect of the rs6495309 C＞T on the risk of COPD was more evident in moderate to very severe COPD than in mild COPD under a dominant model for the variant T allele (P-value of test for homogeneity=0.02). Conclusions: We confirmed the association between the 15q25 region and the risk of COPD in a Korean population.