Adipose Stem Cell Therapy in Mice on Repetitive Intratracheal Bleomycin Induced Pulmonary Fibrosis
의약학 > 내과학
이은주 , 이은정 , 이승현 , 민경훈 , 허규영 , 이승헌 , 이상엽 , 김제형 , 심제정 , 신철 , 인광호 , 강경호 , 이상훈 , 엄창섭 , 김한겸 , 양경숙 , 김현수 , 김용만
대한결핵 및 호흡기학회
대한결핵및호흡기학회 추계학술발표초록집 2012년, 제114권 244(총1페이지)
0y909991.pdf [무료 PDF 뷰어 다운로드]
  • ※ 본 자료는 참고용 논문으로 수정 및 텍스트 복사가 되지 않습니다.
  • 구매가격
    30원 (구매자료 3% 적립)
    이메일 발송  스크랩 하기
    자료 다운로드  네이버 로그인
    Rationale: Adipose-derived stem cells (ASC) are detectable in the parenchyma and large airways of lungs, and associated with reduced inflammatory infiltration and lung cell death. Objectives: We hypothesized that ASC could ameliorate lung fibrosis induced by repetitive intratracheal bleomycin instillation. Methods: Male 8-week-old C57BL/6J mice (control group n=10, bleomycin only group n=20, bleomycin plus adipose stem cell group n=10) were used. Eighth biweekly dose of bleomycin was injected intratracheally via an intubation procedure at dose of 0.04 units in a total volume of 100μl of sterile saline. Human ASC (6×105 cells) were then administered via intra-peritoneal injection every other week during the latter 2 months of the 4- month bleomycin exposure. Lungs were evaluated for histology, collagen content, and TUNEL staining. Bronchoalveolar lavage (BAL) was performed for cell counts. Measurements and Main Results: Biweekly four-times administrations of ASC were associated with reduced hyperplasia of Clara cells, cuboidal AECs with peri-alveolar ducts inflammatory cells infiltration, septal thickening, enlarged alveoli, and extensive fibrosis in response to repetitively intratracheal of instillation of bleomycin. ASC therapy also revealed to suppress TGF- β level. Conclusions: These results suggest a useful therapeutic effect of ASC on pulmonary fibrosis induced by repetitive bleomycin administration. Further studies are needed to evaluate the efficacy of ASC for the treatment of IPF.
    사업자등록번호 220-87-87785 대표.신현웅 주소.서울시 서초구 방배로10길 18, 402호 대표전화.070-8809-9397
    개인정보책임자.박정아 통신판매업신고번호 제2017-서울서초-1765호 이메일
    copyright (c) 2009 happynlife. steel All reserved.