Purpose: Statins, HMG-CoA reductase inhibitors have been shown to inhibit Rho-GTPase signaling, intracellluar signaling molecules involved in epithelial-mesenchymal transition (EMT). Therefore, we investigated whether pravastatin inhibited transforming growth factor (TGF)-β1-induced epithelial-mesenchymal transition in lung cancer cell line. Methods: Cultured adenocarcinomic human alveolar basal epithelial cells (A549 cells) were pretreated with or without 10 uM pravastatin for 24 hours and then were stimulated with TGF-β1, 5 ng/ml for 36 hours. The effect of pravastatin on ex-pression of epithelial phenotypic markers, E-cadherin and mesenchymal phenotypic markers, vimentin was determined by Western blot. Results: Our study confirmed that TGF-β1 induced EMT of A549 cells characterized by cells morphological changes and decrease of expression of E-cadherin, concomitant with increase of vimentin. Inhibition of EMT in A549 cells with pravastatin resulted in preservation of the expression of E-cadherin and in a decrease in vimentin expression. Conclusion: This study shows that pravastatin inhibits the TGF-β-induced EMT of cultured A549 cells. Further studies are needed to elucidate signaling pathway of statins in inhibiting EMT.