Purpose: This study was conducted to investigate how information using leaflet will affect anxiety of inpatients undergoing endoscopic examination. Methods: Non-equivalent control group with a pre-post test design was used. Subjects were selected from inpatients of D hospital in B city for endoscopic examination from May 1 to July 31, 2009. Forty subjects were assigned to either control or experimental group. The Leaflet developed by authors was used as an educational material. Johnson`s visual analogue scale, blood pressure, and pulse were used to measure anxiety. Results: The experimental group informed with the leaflet showed less subjective anxiety than control group without the leaflet (p=.008). Experimental group informed with the leaflet showed less objective anxiety measured by systolic blood pressure, diastolic blood pressure, and heart rate than control group without the leaflet (p=.004, .014, and .009, respectively). Conclusion: Giving information using leaflet to the patient before endoscopy may be applicable as a nursing intervention to reduce anxiety. Further studies are needed to generalize the results of this study.