HPLC-PDA를 이용한 반하, 호장남성, 수반하의 분류 및 함량분석
분야
의약학 > 기초한의학
저자
조지은 ( Ji Eun Jo ) , 이아영 ( A Yeong Lee ) , 김효선 ( Hyo Seon Kim ) , 문병철 ( Byeong Cheol Moon ) , 최고야 ( Go Ya Choi ) , 지윤의 ( Yun Ui Ji ) , 김호경 ( Ho Kyoung Kim )
발행기관
대한본초학회
간행물정보
대한본초학회지(본초분과학회지) 2013년, 제28권 제5호, 95~101페이지(총7페이지)
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35301301.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    Objectives: A quantitative method using high performance liquid chromatography with a photodiode array detector(HPLC-PDA) was established for the quantitative analysis of the four main compound and pattern analysis to classification Piiellia ternate, P. pedatisecta and Typhonium flagelliforme. Methods: The analytical procedure for the determination of P. ternata, together with the known main compounds uracil, uridine, guanosine and adenosine was established. Optimum HPLC-PDA separation of these P. ternata was possible on Luna C18(2) column material, using water and acetonitrile as mobile phase. The method was validated according to regulatory guidelines. In addition, this assay method were analyzed for the content of four main compound in P. ternata, P. pedatisecta and T. flagelliforme and by data obtained from the HPLC-PDA analysis was performed principal component analysis(PCA). Results: Validation results indicated that the HPLC method is well suited for the determination of the roots of P. ternata with a good linearity (r2> 0.999), precision and recovery rates. Analysis of HPLC-PDA, the average content of uracil, uridine, guanosine and adenosine was significantly higher in P. ternate>P. pedatisecta> T. flagelliforme order. The application of PCA to main compound data by HPLC-PDA permitted the effective discrimination among the three species. Conclusions: Analysis of both HPLC-PDA and PCA confirmed the fact that four main compound and pattern profiles of P. ternata, P. pedatisecta and T. flagelliforme were different from each other.
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