The objective of this study is to investigate the effect of elevated CO2 and temperature on the phenology and reproductive ecological characteristics of Bupleurum latissimum. We measured characteristics of 4-year-old B. latissimum grown under control (ambient CO2+ambient temperature, 340~370 ppm) and treatment (elevated CO2+elevated temperature, 690~770 ppm) conditions in a glass house. The period of flowering, fruit appearance and fruit maturing was earlier by 4, 1, 2 days, respectively, under elevated CO2 and temperature. The comparative weight of a single ripe fruit, all ripe fruits and total ripe and unripe fruits was higher per control than treated plants. However the other characteristics, such as no. of peduncle, matured fruits, total fruits per plant and fruit setting rate per plant of B. latissimum were not significantly affected by elevated CO2 and temperature. The germination rate of B. latissimum was higher in control than in treatment plants. These results showed that the phenology of B. latissimuin might be well suited to an earlier growing season, and the response of reproductive characteristics of B. latissimuin is negatively influenced by global warming.