In plastic and reconstructive surgery, reconstruction of bone defect is commonly required in congenital anomaly or trauma. Autogenous bone graft is the most ideal material to reconstruct bone defect. Using the autogenous bone graft, however, is limited due to donor site morbidity and limitation of harvesting. β-tricalcium phosphate (β-TCP) is a homogenous high-purity chemical synthetic material with osteoconductivity. Fibrin forms the environment for fibroblast proliferation and accelerates wound healing. And Lipo-PGE1 promotes vasodilation and prevents platelet aggregation, and induces neovascularization. Thirty-two rabbits were divided 8 groups and 4 bone defects were made on each rabbit`s cranial bone. Bone defects were divided into four groups: control group, β-TCP group, fibrin sealant group, and β-TCP with fibrin sealant group. Lipo-PGE1 was intravenously injected to 16 rabbits, while normal saline was intravenously injected to the others. Results were evaluated by gross, histomorphological and histomorphometrical analysis at 1 week, 2 weeks, 4 weeks, 12 weeks after implantation. Mean percentage of the new bone formation area of β-TCP with fibrin sealant group was statistically significantly higher than that of the other groups. Although there was no statistical significance, mean percentage of the new bone formation area of applying Lipo-PGE1 group was higher than that of not applying Lipo-PGE1 group. We could verify the possibility of β-TCP with fibrin sealant being helpful in new bone formation. And we suggested the possibility of Lipo-PGE1 being helpful in new bone formation.