Optimization of β-Glucosidase Production by a Strain of Stereum hirsutum and Its Application in Enzymatic Saccharification
분야
자연과학 > 생물
저자
( Ramachandran priyadharshini ) , ( Ngoc Phuong Thao Nguyen ) , ( Joon Ho Choi ) , ( Yun Chan Kang ) , ( Marimuthu Jeya ) , ( Jung Kul Lee )
발행기관
한국미생물생명공학회(구 한국산업미생물학회)
간행물정보
Journal of Microbiology and Biotechnology 2013년, 제23권 제3호, 351~356페이지(총6페이지)
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05212228.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    A high β-glucosidase (BGL)-producing strain, Stereum hirsutum, was identified and isolated and showed a maximum BGL activity (10.4 U/ml) when cultured with Avicel and tryptone as the carbon and nitrogen sources, respectively. In comparison with other BGLs, BGL obtained from S. hirsutum showed a higher level of activity to cellobiose (Vmax = 172 U/mg, and kcat = 281/s). Under the optimum conditions (600 rpm, 30oC, and pH 6.0), the maximum BGL activity of 10.4 U/ml with the overall productivity of 74.5 U/l/h was observed. BGL production was scaled up from a laboratory scale (7-L fermenter) to a pilot scale (70-L fermenter). When S. hirsutum was cultured in fed-batch culture with rice straw as the carbon source in a 70-L fermenter, a comparable productivity of 78.6 U/l/h was obtained. Furthermore, S. hirsutum showed high levels of activity of other lignocellulases (cellobiohydrolase, endoglucanase, xylanase, and laccase) that are involved in the saccharification of biomasses. Application of S. hirsutum lignocellulases in the hydrolysis of Pinus densiflora and Catalpa ovata showed saccharification yields of 49.7% and 43.0%, respectively, which were higher than the yield obtained using commercial enzymes.
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