증례 : PCR 증폭과 직접 염기서열분석으로 진단한 Mycobacterium marinum에 의한 피부감염증 1예
분야
의약학 > 피부과학
저자
김진용 ( Jin Yong Kim ) , 서수현 ( Soo Hyun Seo ) , 황은정 ( Sun Jung Hwang ) , 최미라 ( Mi Ra Choi ) , 박성섭 ( Sung Suo Park ) , 섬문우 ( Moon Woo Seong ) , 조광현 ( Kwang Hyun Cho )
발행기관
대한피부과학회
간행물정보
대한피부과학회지 2013년, 제51권 제9호, 734~740페이지(총7페이지)
파일형식
27728967.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    Mycobacterium marinum is an atypical mycobacterium (ATM) and is an uncommon cause of skin and soft tissue infections associated with contact with contaminated water. Diagnosis is often delayed when only a conventional identification method is used. PCR amplification and direct sequencing is recently available method for rapid identification of ATM. We report a case of M. marinum infection identified by PCR and sequencing. A 56-year-old female was referred for multiple erythematous nodules on both forearms which appeared two months ago. Skin biopsy showed suppurative granulomatous inflammation, and AFB culture showed nontuberculous Mycobacteria. PCR and sequencing were performed, and the obtained sequences were compared to the database using BLAST. The sequences of 16S rRNA and rpoB could not differentiate between M. marinum and M. ulcerans, showing 100%homology to both. Identification was possible using the sequences of the tuf and hsp65 genes, showing both 100%homology to M. marinum, while 99.8%, 99.7% to M. ulcerans. The patient was treated with clarithromycin, rifampicin,and ethambutol for 6 months.
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