Two-Step Process Using Immobilized Saccharomyces cerevisiae and Pichia stipitis for Ethanol Production from Ulva pertusa Kjellman Hydrolysate
자연과학 > 생물
( Sang Eun Lee ) , ( Yi Ok Kim ) , ( Woo Yong Choi ) , ( Do Hyung Kang ) , ( Hyeon Yong Lee ) , ( Kyung Hwan Jung )
한국미생물생명공학회(구 한국산업미생물학회)
Journal of Microbiology and Biotechnology 2013년, 제23권 제10호, 1434~1444페이지(총11페이지)
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    We established a two-step production process using immobilized S. cerevisiae and P. stipitis yeast to produce ethanol from seaweed (U. pertusa Kjellman) hydrolysate. The process was designed to completely consume both glucose and xylose. In particular, the yeasts were immobilized using DEAE-corncob and DEAE-cotton, respectively. The first step of the process included a continuous column reactor using immobilized S. cerevisiae, and the second step included a repeated-batch reactor using immobilized P. stipitis. It was verified that the glucose and xylose in 20 L of medium containing the U. pertusa Kjellman hydrolysate was converted completely to about 5.0 g/l ethanol through the two-step process, in which the overall ethanol yield from total reducing sugar was 0.37 and the volumetric ethanol productivity was 0.126 g/ l/h. The volumetric ethanol productivity of the two-step process was about 2.7 times greater than that when P. stipitis was used alone for ethanol production from U. pertusa Kjellman hydrolysate. In addition, the overall ethanol yield from glucose and xylose was superior to that when P. stipitis was used alone for ethanol production. This two-step process will not only contribute to the development of an integrated process for ethanol production from glucoseand xylose-containing biomass hydrolysates, but could also be used as an alternative method for ethanol production.
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