REVIEW ARTICLE : Evaluation and Application of Cryosectioning in Undecalcified Hard Tissues in Cartilage and Bone Regenerative Medicine
의약학 > 의공학
( Seung Hwa Park ) , ( Jee Won Yoon ) , ( Jae Hyun Park ) , ( Bo Young Kim ) , ( Judee Grace E Nemeno ) , ( Ernesto C Balolong Jr ) , ( Yuna Han ) , ( Kyung Mi Lee ) , ( Soo Jung Lee ) , ( Jeong Ik Lee )
조직공학과 재생의학 2015년, 제12권 제2호, 94~104페이지(총11페이지)
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    This article overall describes the development of histological method especially, cartilage and bone research in regenerative medicine. Kawamoto’s film method, which has been recently introduced into our laboratory, has been applied in various fields that make sectioning of hard tissues easier than the conventional method. Moreover, this method also does not require the time-consuming chemical fixation and/or decalcification process. Kawamoto method involves the use of the adhesive plastic film instead of a cover glass where the thin tissue sections are attached efficiently at low temperatures (-25°C). Furthermore, the histological method preserves the enzymatic activity in the fresh sections in comparison to that of chemically-treated tissue sections. In fact, we used this Kawamoto’s film method in one of our researches in which drug repositioning was employed for cartilage regeneration. Some of the Kawamoto-processed tissue sections are featured in this review article. Therefore, the application of this tissue preparation technique allowed effective and histological and histochemical studies within a shorter preparation time with ease and convenience. In the future, this Kawamoto method may offer further applications in the preparation of a more diverse tissues and samples not only in the preclinical but also in the clinical studies. Tissue Eng Regen Med 2015;12(Suppl 2):94-104
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