Purpose: Traumatic brain injury (TBI) is a significant cause of morbidity and mortality worldwide. Severity of the initial insult is one of the most significant factors affecting outcome following TBI. In order to investigate the mechanisms of cellular injury and develop novel therapeutic strategies for TBI, we designed a standardized animal TBI model and evaluated histological and functional outcomes according to the degree of impact severity.
Methods: Male adult C57Bl/6 mice underwent controlled cortical impact (CCI) at varying depths of deflection (1.0-2.0 mm). We performed hematoxylin and eosin staining at 7 days after recovery from TBI. Neurobehavioral characterization after TBI was analyzed by the Barnes maze test, passive avoidance test, open field test, rotarod test, tail suspension test, and light/dark test.
Results: We observed a graded injury response according to the degree of deflection depths tested (diameter, 3 mm; velocity, 3 m/s; and duration, 500 ms) compared to sham controls. In the Barnes maze test, the severe TBI (2 mm depth) group showed reduced spatial memory as compared with the sham and mild TBI (1 mm depth) groups at 7 days after TBI. There was a significant difference in the results of the open field test and light/dark test among the three groups.
Conclusion: Our findings demonstrate that the graded injury responses following TBI resulted in differential histopatho-logical and behavioral outcomes in a mouse experimental CCI model. Thus, a model of CCI with histologic/behavioral outcome analysis may offer a reliable and convenient design for preclinical TBI research involving mice.
[의학] tbi[외상성 뇌손상]에 관해
[뇌질환][외상성뇌손상][뇌혈류장애][뇌졸중][노인성뇌질환][신경질환]뇌질환과 외상성뇌손상, 뇌질환과 뇌혈류장애, 뇌질환과 뇌졸중, 뇌질환과 노인성뇌질환, 뇌질환과 뇌심혈관계질환, 뇌질환과 신경질환 분석
[외상성뇌손상, TBI] 외상성뇌손상(TBI)의 정의, 증상, 분류, 환자의 분류와 외상성뇌손상(TBI)의 진단 및 외상성뇌손상(TBI)의 치료 심층 분석
간호진단 응급실 외상성 뇌손상 케이스 traumatic brain injury
간호학 외상성 뇌손상 분류 진단적 검사
[신경해부학][신경로][뇌][외상성뇌손상][척수병변][해부학][신경][신경해부]신경해부학과 신경로, 신경해부학과 뇌, 신경해부학과 외상성뇌손상, 신경해부학과 척수병변 분석(신경해부학, 신경로, 뇌,외상성뇌손상)
[뇌손상] 뇌손상 재활[Rehabilitation of TBI], 뇌손상의 종류와 원인 및 증상, 또는 치료에 관해
[뇌손상] 외상성 뇌손상 TBI[Traumatic Brain Injury]에 관해
뇌병변장애(뇌성마비, 외상성 뇌손상, 뇌졸중)
간호학 외상성 뇌손상 치료와 간호