HOME > 의약학 > 내과학 > 대한간학회 > 춘·추계 학술대회 (KASL)
O-011 : EpCAM-High Liver Cancer Stem Cells Resist Natural Killer Cell-Mediated Cytotoxicity by Upregulation of Cell Surface CEACAM1
분야 의약학 > 내과학
저자 ( Pil Soo Sung ) , ( Dong Jun Park ) , ( Yuji Choi ) , ( Jung-hee Kim ) , ( Wonhee Hur ) , ( Seung Kew Yoon )
발행기관 대한간학회
간행물정보 춘·추계 학술대회 (KASL) 2017년, 제2017권 제1호, 22(총1쪽)
파일형식 27410164.pdf [다운로드 무료 PDF 뷰어]
판매가격 1,000원
적립금 30원 (구매자료 3% 적립)
이 자료를
논문의 미리보기 2페이지를 PDF파일로 제공합니다.
 
연관 논문
PO-073 : UBE2T: A Molecular Regulator for Cancer Stemness in Hepatocellular Carcinoma
PE-152 : UBE2T: A Molecular Regulator for Cancer Stemness in Hepatocellular Carcinoma
C-terminal truncated HBx reduces doxorubicin cytotoxicity via ABCB1 upregulation in Huh-7 hepatocellular carcinoma cells
PE-147 : Profiling of Secretory Proteins from Human Bone Marrow- Derived Mesenchymal Stem Cells in Liver Repair by cDNA Microarray
Comparative Study on the Content and Cytotoxicity of Pseudolaric Acid B in the Five Plant Parts of Pseudolarix kaempferi
 
 
영문초록
Aims: The major malignant phenotypes of cancer, including recurrence, metastasis, and chemoresistance, are related to the presence of cancer stem cells (CSCs). Epithelial Cell Adhesion Molecule (EpCAM) is a type 1 transmembrane protein that is detectable in hepatic stem/progenitor cells and cholangiocytes. Natural killer (NK) cells are the prototype innate lymphoid cells endowed with potent cytolytic function that provide host defense against microbial infection and tumors. However, there are conflicting results: on the cytotoxicity of NK cells against cancer stem cells. In this study, we investigated whether NK cells exhibit enhanced or decreased cytotoxicity against EpCAM+ liver CSCs and the underlying mechanism of the phenomenon. Methods: EpCAMhigh and EpCAMlow Huh-7 cells were sorted by flow cytometry. Human NK cells were isolated by magnetic sorting from peripheral blood mononuclear cells of healthy donors. NK cell and hepatoma cells were co-cultured and cytotoxicity assay was performed. Immunohistochemical staining was performed using human HCC tissues obtained from surgical specimens from the patients who underwent liver resection.Results: Patients with positive EpCAM expression in their tumors showed higher serum α-fetoprotein levels and histological Ki-67 expression. The frequency of early and massive recurrence was higher in patients with positive EpCAM expression in their tumors. Co-culture experiment demonstrated that EpCAMhigh Huh-7 cells resisted NK cell-mediated cytotoxicity. We have screened the potential activating or inhibiting ligands of NK cell receptors, and identified that carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1), which is known to inhibit NK-cell-mediated killing, was upregulated on the surface of EpCAMhigh Huh-7 cells. Silencing CEACAM1 restored cytotoxicity of NK cells against EpCAMhigh Huh-7 cells, suggesting that CEACAM1 is responsible for attenuated NK cell-mediated killing of these cells.Conclusions: Overall, our data clearly demonstrate that EpCAMhigh liver CSCs resist NK cell-mediated cytotoxicity by upregulation of cell surface CEACAM1 expression.
 
 
Hepatocellular carcinoma, EpCAM, Cancer stem cell, Natural killer cell
 
 
도움말
본 논문은 참고용 논문으로 수정 및 텍스트 복사가 되지 않습니다.
 
 
추천자료
[PBL] 전신부종과 호흡곤란을 호소한 젊은 여자
[병리학 논문분석] PDL cell과 LPS와 nicotine 처리했을때의 VEGF 발현에 관한 연구
[[솔루션]] :: Lehninger Biochemistry 5edition[the foundations of Biochemistry]
[발생공학] 배아 줄기 세포 연구(영문)
재생불량성빈혈
국가공무원 영어기출문제(1993~2006년 9급,7급,국회8급기출문제집)
[PBL] AIH(Autoimmune hepatitis)(영문)
혈액의 구조와 기능
[토익]영어단어 보카33000
[병리학] Paclitaxel-resistant 암세포에 민감성을 갖는 천연물질을 선별 및 항암기전 연구
오늘 본 자료
오늘 본 자료가 없습니다.
장바구니 담은 자료
장바구니가 비어 있습니다.
이 간행물 인기자료
최신 간암 치료제 Update
간암에서 인터벤션 치료의 실제
간암 가이드라인 어떻게 적용할 것인...
간세포암종의 영상학적 소견-CT, MR을...
간암검진에서의 초음파 영상 촬영과 ...
이 간행물 신규자료
Molecular Targets for Nonalcoholic ...
Hepatocellular Carcinoma Developmen...
Noninvasive Diagnostic Method of No...
Lipid Droplet as a Potential Target...
Liver Resection Using Robotic Syste...
저작권 정보
본 학술논문은 한국학술정보㈜ 각 학회간에 저작권 계약이 체결된 것으로 HAPPY학술이 제공하고 있습니다. 본 저작물을 불법적으로 이용시는 법적인 제재가 가해질 수 있습니다.
 
서비스이용약관 | 개인정보취급방침 | E-mail 수집 거부 | 제휴 및 광고문의 | FAQ
이메일 무단 수집 거부
본 웹사이트에 게시된 이메일 주소가 전자우편 수집 프로그램이나 그 밖의 기술적 장치를 이용하여 무단으로 수집되는 것을 거부하며, 이를 위반시 정보통신망법에 의해 형사처벌됨을 유념하시기 바랍니다. [게시일 2003년 4월 2일]