Generation of heterologous proteins-expressing recombinant snakehead rhabdoviruses (rSHRVs) using reverse genetics

분야: 수해양 > 수산생물학
저자: ( Jun Soung Kwak ) , ( Sujeong Ryu ) , ( Ki Hong Kim )
발행기관: 한국어병학회
간행물정보: 한국어병학회지 2020년, 제33권 제2호, 163~169페이지(총7페이지)
파일형식: 92801035.pdf [무료 PDF 뷰어 다운로드]
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영문초록

Snakehead rhabdovirus (SHRV) is different from other fish novirhabdoviruses such as viral hemorrhagic septicemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), and hirame rhabdovirus (HIRRV) in that it replicates at high temperatures. Therefore, the delivery of foreign proteins to fish living at high water temperature would be possible by using recombinant SHRVs. In the present study, to evaluate the possible use of SHRV as a vehicle for foreign proteins delivery, we generated a recombinant SHRV that contains an enhanced-GFP (eGFP) gene between nucleoprotein (N) and phosphoprotein (P) genes (rSHRV-A-eGFP), and another recombinant SHRV expressing two heterologous genes by inserting an eGFP gene between N and P genes, and mCherry gene between P and M genes (rSHRV-AeGFP-BmCherry). Epithelioma papulosum cyprini (EPC) cells infected with the recombinant SHRVs showed strong fluorescence(s), suggesting the possible availability of recombinant SHRVs for the development of combined vaccines by expressing multiple foreign antigens.

#Recombinant snakehead rhabdovirus # rSHRVs # Reverse genetics # Delivery of heterologous genes

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