Anaerobic fermentation using Clostridium butyricum NCIB 9576, and photo-fermentation using Rhodopseudomonas sphaeroides E15-1 were studied for the production of hydrogen from Makkoli, fruits (orange & apple, watermelon & melon) and Tofu wastewaters. From the Makkoli wastewater, which contained 0.94 g/? sugars and 2.74 g/? soluble starch, approximately 49 mM H?/? wastewater was produced during the initial 18h of the anaerobic fermentation with pH control between 6.5-7.0. Several organic acids such as butyric acid, acetic acid, propionic acid, lactic acid and ethanol were also produced. From Watermelon and melon wastewater, which contained 43 g/? sugars, generated about approximately 71 mM H?/? wastewater was produced during the initial 24 h of the anaerobic fermentation. Tofu wastewater, pH 6.5, containing 12.6 g/? soluble starch and 0.74 g/? sugars, generated about 30 mM H?/? wastewater, along with some organic acids, during the initial 24h of anaerobic fermentation. Makkoli and Tofu wastewaters as substrates for the photo-fermentation by Rhodopseudomonas sphaeroides E15-1 produced approximately 37.9 and 22.2 ?M H?/? wastewaters, respectively for 9 days of incubation under the average of 9,000-10,000 lux illumination at the surface of reactor using tungsten halogen lamps. Orange and apple wastewater, which contained 93.4 g/l, produced approximately 13.1 ?M H?/? wastewater only for 2 days of photo-fermentation and the growth of Rhodopseudomonas sphaeroides E15-1 and hydrogen production were stopped. |