각 복사하기 버튼을 이용하여 블로그나 카페에 붙여넣기 하실 수 있습니다.
 
분야 : 자연과학 > 생물
발행기관 : 한국미생물생명공학회(구 한국산업미생물학회)
간행물정보 : Journal of Microbiology and Biotechnology, 2012 pp.1258~1263 (총 6pages)
 
 
영문초록
2, 3-Butanediol (2, 3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2, 3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2, 3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene to generate plasmids pSB1-7. The plasmids were then introduced into K. pneumoniae using electroporation. All strains were incubated in flask experiments and 2, 3-BD production was increased by 60% in recombinant bacteria harboring pSB04 (budA and budB genes), compared with the parental strain K. pneumoniae KCTC2242. The maximum 2, 3-BD production level achieved through fedbatch fermentation with K. pneumoniae SGJSB04 was 101.53 g/l over 40 h with a productivity of 2.54 g/l.h. These results suggest that overexpression of 2, 3-BD synthesisrelated genes can enhance 2,3-BD production in K. pneumoniae by fermentation.
 
 
2, 3-Butanediol, Klebsiella pneumoniae, gene manipulation, acetolactate decarboxylase, acetolactate synthase, alcohol dehydrogenase
 
다운로드 05212019.pdf