[유전학] Enhancing the Efficiency of Transgene Expression(영문)

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[유전학] Enhancing the Efficiency of Transgene Expression(영문)에 대한 자료입니다.
목차
-Introduction

-Regulation of Transgene Insertion

-Regulation of DNA level

-Regulation of RNA level

-Application to livestock

-Conclusion

-References
본문내용
Introduction

BUT, How can we enhance
the Efficiency of Transgene Expression?

Regulation of Transgene Insertion

ZFN : fok1 restrict enzyme + Zinc Finger Protein
Fok1 restrict enzyme : recognition domain andnuclease domain are separated.
Zinc Finger Protein can recognize 9 base pairs.

ZFN can recognize 9 base pairs for each side by dimerization.

Specific sites can be cleaved by ZFN.


1. Promoter
Promoter is composed of
TF binding site and RNP binding site.

Strong promoter→Acceleration of TF binding →Acceleration of RNA polymerase binding
→Enhancing expression

CMV is not the always strong promoter.
Thus, choose the proper promoter for different cells.
참고문헌
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