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분야
ppt2.채취방법
3.객담의 그람염색
4.Legionella pneumophila
5.Mycobacteria tuberculosis
6.Fluorescent AFB stain
7.고체배지법과 액체배지법 비교
8.MTB indentification & PCR
냉각탑수, 에어컨 등의 응결수 등에 존재
L. pneumophila serogroup 1-6에 의한 호흡기 감염이 흔함
재향군인병(Legionnaires disease)
폰티악 열(Pontiac fever)
무증상 감염
항산성 염색 (Acid fast stain)
Ziehl-Neelsen stain, Kinyoun stain
Direct stain: Sensitivity 42%
Concentrated stain: Sensitivity 74%
Fluorescent AFB stain: Auramine-rhodamine stain, 80%
배양법 (Gold standard)
고체 배지법: 계란배지, 한천배지 (8주)
액체 배지법: MGIT, Bact/ALERT 3D (6주)
PCR: rapid, sensitivity 90-95%, expensive
Tuberculin reaction, radiologic test
Quantiferon assay
TB culture: sensitive, slow growing (8 weeks)
Identification
Niacin test: 3 days – 2 weeks, false negative
DNA probe method: 1 day, expensive
PCR indentification: rpoB gene amplification
1 day, not expensive
검체의 resampling 없는 TB PCR
AFB stain 양성 검체 2주간 보관
PCR 처방시 AFB 양성인 검체로 시행
