Bioethanol is one of the alternative sources of oil resources which received attention with biodiesel. Many countries produce bioethanol from food sources such as corn and sugarcane, but it causes food-energy conflicts and farmland problem. So it needs to focus on new biomass which is sustainable, renewable, cost-effective and do not occurred any conflicts. Algae are one of the bioma
ethanol을 얻기 위한 separtion공정이다.
① D1 unit (distillation)
원할한 azeotropic distillation을 하기 위하여 별도의 Distillation 유닛을 달아 separartion model로 들어오는 feed stream을 water와 ethanol의 azeotrope point 조성으로 맞추어 주었다.
그림 53. D1 unit
mole fraction of Azeotrop
process and remove the moisture generated in the fermentation process of alcohol distillation through azeotropic distillation, extractive distillation, the distillation process of these kinds are used. How to manufacture in order to overcome the disadvantages of simple distillation separation process difficult to concentrate with an absorbent such as zeolite, anhydrous ethanol has been studied.
of acetone, 1ml of benzldehyde and 2ml of EtOH.
2) To this mixture, 1.5ml of 10% aqueous NaOH is slowly added by a oil bath keeping temperature at 25~30℃.
3) The resulting reaction mixture is allowed to proceed at rt for 30 min and then 40℃ for 1h.
4) After cooled to rt, the reaction mixture is extracted with diethyl ether(20ml, water 15ml)
5) The organic phase is washed with 20% aqueous NaHS
that has the selection antibiotic. The bacteria are centrifuged, and a buffer resuspends the pellet. Second, the strong alkali, usually sodium hydroxide, and detergent, usually sodium dodecyl sulfate, lyse the bacteria. Finally, potassium acetate neutralizes the strong alkaline condition.
1.5 mL tube
Alkaline lysis solution 1, 2, 3
TE with a pH of 8.0