The RAD3 gene of Saccharomyces cerevisiae is essential for the incision step of UV-induced excision repair. An yeast RAD3 gene has been previously isolated by functional complementation. In order to identify the RAD3 homologous gene from fungus Copr
Base excision repair
Nucleotide excision repair
DNA polymerase 오류로 잘못 짝지어진 염기 복구
MutH, MutL, MutS 단백질
DNA helicaseⅡ, SSB
DNA polymerase Ⅲ
유전성 비용종성 대장암(hereditary nonpolyposis colon cancer, HNPCC)
gene directly by mutating it. This is done just in case the mutation of xisC is not sufficient to prevent formation of hupL subunit.
For two of our approaches, 10 bases are deleted for the mutations to take place
The method we are going to use is “Homologous recombination” to knock out hupL and xicS genes. Homologous recombination is type of genetic recombination which DNA s
gene을 deletion 시켜 보고, 또 over-expression 시켜볼 것이다. 그리고 그러한 genetic manipulation이 yeast의 phenotype에 어떠한 변화를 유발하는지 알아보고, 그로부터 Stp1, TOR kinase의 역할에 대해 생각해 볼 것이다. Homologous recombination을 이용한 yeast chromosomal gene의 deletion, check PCR, expression vector를 이용한 over-expression의
genesis, wound healing, and the immune and inflammatory response. Leptin acts through the leptin receptor (LEPR; 601007), a single-transmembrane-domain receptor of the cytokine receptor family, which is found in many tissues in several alternatively spliced forms. The LEP gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.
1. 실험 제목
Bioinformatics & Sequencing
sequencing에 대해 이해하고, sequencing을 통해 알 수 있는 DNA 염기서열을 가지고 BLAST 분석을 통하여 gene의 ORF, homology, restriction enzyme site, motif, 2D structure 등을 알아보고 한다.
3. 실험 방법
① 실험 수업 시간에 실험 조교가 Nucleotide blast, Protein blast, Homolog