viability을 구했다. 이를 통해 ethanol의 농도에 따른 cellviability의 관계를 알아냈고 IC50을 구하였다
대조군은 3번 반복, 처리군은 2번 반복한 평균값을 이용하였다.
동물모델에서의 독성평가: 경구투여에 의한 독성 평가
G-bio에서 구입한 흰 쥐(mice)를 8-9주간 실험실에서 사육한 뒤 실험에 사용하였
날짜: day 1 (11/15)
실습명: 세포분양, Media preparation, dilution
실습 목적:Attached cell culture를 하기 위해 세포를 분양 받는다. 세포를 배양하기 위한 complete media를 제조한 후 cell과 적절하게 dilution하여 suspended cell culture를 시작한다.
실험에 사용한 도구:DMEM(Eagle’s minimal essential medium)complete media(1% penicillin/
is indicated
It often affects the ascending aorta and aortic arch, causing obstruction of the aorta and its major arteries
Pulseless disease
: frequent occlusion of the large arteries originating from the aorta
The pathology
: panarteritis, characterized by mononuclear cells and occasionally giant cells, with intimal hyperplasia, medial and adventitial thickening
Mid-1800s - Beginning research of Embryo
1908 – ‘Stem Cell’ from Alexander Maksimov
1963 – Self reproducing cell
1978 – Found Blood stem cell
1981 - Embryonic stem cell
1998 - Human's embryonic stem cell
2001 – First human embryo cloning
(중략)
2.1 Adult Stem Cell - How?From Bone Marrow
HSCs and MSCs are rich in b
1. Bacteria(E.coli)
Advantage
Fast Growth rate
Cheap
Various cloning vectors
Disadvantage
Impossibility of posttranslational modification
Inclusion body
Only simple proteins
2. Yeast(S.cerevisiae)
Advantage
Large scale process
Cheap
partially possibility of posttranslational modification
Disadvantage
Shortage of cloning vectors
Overglycosylation