The luminol is activated with an oxidant. Hydrogen peroxide solution and sodium hydroxide is
used as the activator. In the presence of an catalyst, the hydrogen peroxide is decomposed to form
oxygen and water. The iron in potassium ferricyanide is a catalyst for the reaction.
When luminol reacts with the sodium hydroxide, a dianion is formed. The oxygen produced from
the hydrogen peroxi
In synthetic luminol process we need luminol’s precursor. That is
5- nitrophthalhydrazide. It is synthesized by the reaction of 3-nitrophthalic acid
with hydrazine. This process is condensation reaction. First, hydrazine(N2H4) is
heated with the 3-nitrophthalic acid. This reaction is most conveniently carried
out in the high-boiling solvent triethylene glycol. To boil off excess water, t
In catalyst, oxygen produced from H202
With sodium hydroxide, luminol Dianion is formed
The oxygen reacts with luminol dianion
This product is very unstable, so decomposes to 5-aminophathalic acid
As the exited state, 5-aminophathalic acid relaxes to ground state
Excess energy is emitted as blue light
Alkaline luminol, H2O2 solution is used on detecting NO2
The presence o
chemiluminescence, electro chemiluminescence 등의 method가 사용되는데, 그 중 가장 많이 사용되는 것은 colorimetric detection이다.
(1) Colorimetric assay
Colorimetric method는 color의 변화를 통해 특정 chemical을 detection하는 방법이다. Color signal을 육안으로 쉽게 식별할 수 있으며, 단순한 관찰만으로도 detection 가능하다는 이점