Digestion tank production gas is a biogas produced by artificial anaerobic digestion of organic wastes with bacteria in the digestion tank. This can be divided into mergence gas which produce bio-gas by putting more than two of them together, among the organic waste gas, waste water gas, livestock· sewage gas, sewage sludge gas, depending on the type of organic waste. Because they are artificial
Stomach
Hydrochloric acid
Prepares protein for digestion
Activates enzymes
Pepsin
Begins protein digestion
Gastric lipase
Some fat digestion
Gastrin (hormone)
Stimulates gastric secretion and movement
Intrinsic factor
Needed for absorption of vitamin B12
Small intestine
Sections of small intestine
Duodenum, jejunum, ileum
Digestion
Bicarbonate neutralizes stomach acid
Pancreatic
Evaluating protein quality
Amino acid composition
Digestibility
Protein Digestibility-Corrected Amino Acid Score (PDCAAS)
Used to determine %DV
Types of vegetarian diets
Semi-vegetarian
Usually includes fish, poultry; no red meat
Lacto-ovo vegetarian
Includes milk, eggs
Vegan
Plant foods only
Dietary recommendations
Choose a variety of foods
Choose whole, unrefined foods
Choose a
1. Date
Day 1 – 2008년 10월 27일 월요일
2. Subject & Purpose
Digestion of DNA with Restriction Enzyme & Agarose Gel Electrophoresis & Gel Purification
Plasmid pET vector와 FabG gene이 insert된 pET vector를 두 가지 제한효소, BamHⅠ과 NdeⅠ으로 자른 다음 전기영동을 통해 결과를 확인하고 Purification을 한다.
3. Principle
1) Restriction enzy
Structure
Glycerol + two fatty acids + phosphate group
Functions
Component of cell membranes
Lipid transport as part of lipoproteins
Emulsifiers
Food sources
Egg yolks, liver, soybeans, peanuts
Mouth and stomach
Minimal digestion of triglycerides
Small intestine
Emulsified by phospholipids
Digested by pancreatic lipase
Absorbed into intestinal cells
Formed into chylomicrons and move
Digestion of DNA with Restriction Enzyme
I. Apparatus
- Water bath, brief centrifuge
II. Reagents
- Plasmid, DDW, restriction enzymes [NdeI, BamHI], restriction enzyme reaction buffer
① NdeI: an endonuclease isolated from Neisseria denitrificans. In molecular biology, it is commonly used as a restriction enzyme.
② BamHI: a restriction enzyme, derived from Bacillus amyloliquefaciens.
2. 2. Extraction
After the fermentation, the microorganisms(Alcaligenes latus) are filled with PHB in their body. These PHBs have to be separated from microorganisms to make final product. To separate PHBs from biomass, they are extracted from biomass by using solvent. These PHB extracted solvent will experience washing and drying process. After that, PHB can be precipitated as white powder.