2. 2. Extraction
After the fermentation, the microorganisms(Alcaligenes latus) are filled with PHB in their body. These PHBs have to be separated from microorganisms to make final product. To separate PHBs from biomass, they are extracted from biomass by using solvent. These PHBextracted solvent will experience washing and drying process. After that, PHB can be precipitated as white powder.
PHB`s Separation & Purification
Target of this study conducted to obtain a purely material PHB purification process debris in the damaged cells, and to remove the remaining medium components. PHB has been removed during the refining process with just remove the present efficiency of the overall purification stayed in the 70-80%.
PHB Synthesis Pathway
Alcaligenes eutrophus PHB in normal circ
from propylene.
After several processes.
Products we have are 40% of acetone and water solution.
How we get nearly pure acetone?
Factors to be considered:
Feasibility
Product value
Cost
Product quality
selectivity
Possible Process:
Distillation
Extraction
Extractive distillation
Extraction(1)+Distillation
Extraction(2)+Distillation
Mainly involved in separation process
-Extract
like hydrogen fueled car. However, although our interest in fuel cell is increasing, the stage of the development is still slight. To develop fuel cell technique as the core industry, we have to construct effective infrastructures fromextraction to supply. So, in this project, our team has searched the fuel cell technique dividing into four parts ; extraction, storing, type, merits & demerits.
Low-melting temperature (LMT) – 저온에서 녹는 특별한 agarose사용
Electroelution – 전류를 통해 DNA를 분리하는 방법
1) Dialysis tubing : dialysis tubing 통해 DNA분리 방법
2) NA-45(nitrocellulose)membrane – Gel 조각에 NA-45membrane붙이고 전기장 주어 분리하는 방법
High-salt extraction – 고농도 Na+에 의해 agarose g