2) when cells are activated by mechanical trauma, cytokines and growthfactors, etc. Conversionof arachidonic acid to prostanoid endproducts is carried out by cyclooxygenases (COX), also known as prostaglandin H synthase (PGHS), at two different sites on the enzyme.
Arachidonic acid is initially cyclized and oxidized to the endoperoxide PGG2 at the cyclooxygenase site of the COX and the product
of peptide that cause stimulation of target organ should be the same
⑤ The assay must not measure biologically inactive degradation product
⑥ It should be possible to prevent the physiologic response by removing the peptide from the blood or by specific antagonism at its receptor
1.Gastrin
Action
major hormonal stimulant of gastric acid secretion
trophic action to gastric mucosa
Recombinant Hormone
Recombinant hormone
Recombinant DNA기술에 의해 합성된 호르몬
진핵세포주 호르몬 생산의 장점
Post Translational Modification
Protein folding
Glycosylation
Codon usage bias
Post Translational Modification
진핵세포에서만 일어남
Chaperon 구조체에 의해 단백질 접힘
당단백질 부착 과정
Glycosylation
Codo
2. Material and method
2.1 실험 동물
시험에 사용한 동물은 체중 30g내외의 흰쥐로, 15마리를 공급받아 3시간 동안 실온에서 굶긴 후 실험에 사용하였다. 각 cage 당 대조군(A) 5마리, 글루코스를 투여할 실험군B, streptozotocin을 투여할 실험군C 를 각각 5마리를 넣어 두었으며, 물과 사료를 자유로이 섭취하도