1.1.2. Cyclooxygenase-prostanoid cascade
Figure 1.1. Metabolism of arachidonic acid
Despite the diverse chemical structure of aspirin-like drugs, the antinociceptive effect of NSAIDs is mainly due totheir common property of inhibiting cyclooxygenases involved in the formation of prostanoids. Prostanoids areformed by most cells and act as autocrine and paracrine lipid mediators. They are n
DNA Repair
Mismatch repair
Base excision repair
Nucleotide excision repair
Direct repair
Mismatch repair
DNA polymerase 오류로 잘못 짝지어진 염기 복구
효소와 단백질
MutH, MutL, MutS 단백질
DNA helicaseⅡ, SSB
Exonuclease Ⅰ
DNA polymerase Ⅲ
DNA ligase
유전성 비용종성 대장암(hereditary nonpolyposis coloncancer, HNPCC)
전체
Stem cell calssification
1. Based upon location
Embryonic stem cells
Somatic/ adult stem cells
2. Based upon function
Totipotent
Pluripotent
Multipotent
Unipotent
Reject Response
Traditional Immuno-repressents
Constant administration
Little engraftment after 3~4 weeks
Host immune system impaired
Vulnerable to Infection
↓Need brief immuno-re
humancancercells.
Treated Methanol extracts from Linum usitatissimum and Perilla frutescens to human gastric adenocarcinoma cells, humancolon adenocarcinoma cells, and human hepatocellular carcinoma cells
Result
- Methanol extracts from Perilla frutescens showed stronger antimutagenic activity
- Methanol extract from Linum usitatissimum show more effectiveness for growth inhibiti
3. 암화에 의한 세포주 확립
동물세포를 이용한 연구 발전에 기여
4. Sato등에 의해 무혈청 배양법 확립
1960. 무혈청 배양 시도
1963-5(Ham) 혈청 공급 없이
CHO cell cloning
1970(Sato) 혈청 대신 호르몬
첨가 cellline 무혈청 배양
성공
Human embryonal kidney, SV40 and large T antigen transformed