Abstract
Bioethanol is one of the alternative sources of oil resources which received attention with biodiesel. Many countries produce bioethanol from food sources such as corn and sugarcane, but it causes food-energy conflicts and farmland problem. So it needs to focus on new biomass which is sustainable, renewable, cost-effective and do not occurred any conflicts. Algae are one of the bioma
2. 2. Extraction
After the fermentation, the microorganisms(Alcaligenes latus) are filled with PHB in their body. These PHBs have to be separated from microorganisms to make final product. To separate PHBs from biomass, they are extracted from biomass by using solvent. These PHB extracted solvent will experience washing and drying process. After that, PHB can be precipitated as white powder.
extraction 시 사용되는 phenol의 pH : 7.9(RNA 시는 4.5)
Chloroform
Phenol이 작용시 적은 양이나마 수용액에 녹는다. 이렇게 녹은 phenol을 수용액에서 제거 및 Interphase가 잘 형성되게 해준다
Isoamyl alcohol
실험 시 거품이 생기는 거 방지 및 Interphase 형성 도움
Phenol : Chloroform : Isoamyl alcohol = 25 : 24 : 1 또는
Phenol :
1. Introduction
1) Definition of preservation and food-spoilage micro-organisms
Food preservation is the process of treating and handling food to stop or greatly slow down spoilage (loss of quality, edibility or nutritive value) caused or accelerated by micro-organisms. Some methods, however, use benign bacteria, yeasts or fungi to add specific qualities and to preserve food (e.g., cheese, wine
1. 정확한 위치에서의 DNA 절단: 염기서열에 특이적인 restriction endonucleases는 클로닝에 필수적인 분자 가위 역할을 한다
2. 자기 자신을 복제할 수 있는 작은 DNA 분자를 선택: 이러한 DNA를 cloning vector라고 한다. Vector는 운반체이며 전형적으로 플라스미드 또는 바이러스의 DNA가 있다.
3. 두 DNA 조각을 공유