protein은 lipid-detergent mixed micelle을 형성하고 transmembrane protein은 protein-lipid-detergent complex를 형성한다. 이렇게 micelle을 형성하는 정도를 CMC (critical micelle concentration)라 하는데 이것은 고효율 및 고순도의 단백질 정제에 중요한 역할을 하며 pH, 온도, ionic strength, multivalent ions of organic solvents, 및 detergent 순도등
Protein등와 함께 침전되고 Plasmid는 상층액에 속함
Phenol : 수용액에 녹아 있는 단백질을 제거(endonuclease)
Phenol 안에는 8-hydroxyquinoline이란 물질이 들어 있고, 산화 시 붉은 색을 띄게 됨
DNA extraction 시 사용되는 phenol의 pH : 7.9(RNA 시는 4.5)
Chloroform
Phenol이 작용시 적은 양이나마 수용액에 녹는다. 이렇
1. Introduction
1) Definition of preservation and food-spoilage micro-organisms
Food preservation is the process of treating and handling food to stop or greatly slow down spoilage (loss of quality, edibility or nutritive value) caused or accelerated by micro-organisms. Some methods, however, use benign bacteria, yeasts or fungi to add specific qualities and to preserve food (e.g., cheese, wine
I. ProteinExtraction – Micro Smash MS-100 equipment 사용
<실험 전 준비>
얼음(정사각형 스티로폼), E-tube 총 38개(8개×4세트->extract 옮길 것(sonication), centrifuge 상층액 뜰 것, 정량할 것, western 용/ 6개-> standard곡선 그릴 것), screw tube(cap-tube, 뚜껑 초록색) 8개
1. E-tube에 bead (큰 것 2개, 작은 것 10개) 넣어서 ice-rack에
1. Heart disease & cardiac enzymes
1.1 heart disease
Team#5 has had researches about diagnosis of cardiovascular disease using cardiac marker (cardiac enzyme). Cardiovascular disease (heart disease) is a class of diseases that involve the heart or blood vessels (arteries and veins). Cardiovascular diseases remain the biggest cause of deaths worldwide, through over the last two decades, cardio