* Omalizumab (Xolair) is the 149 kDa, glycosylated antibody which is produced by an engineered CHO cell line. This recombinant humanized monoclonal antibody blocks the high-affinity Fc receptor of immunoglobulin E (IgE). It is used to treat patients with moderate-persistent to severe-persistent asthma.The product was approved in the EU in 2005, although it has been available in the US since 2
a sticky end. One of the earlier enzymes to be used, it is popular for historical reasons, but also because digestion leaves a GATC overhang compatible with many other enzymes. Persistent issues with this specific enzyme spontaneously failing to generate cloneable fragments necessitate aliquoting prior to use. Engineered variants of the enzyme have been created in order to avoid star activity.
HEK 293 EBNA1 cell
Freestyle medium
293 SFM II medium
Enriched medium (293 SFM II medium + 5X amino acid solution)
E.coli cell 안에서 형질전환 된 pTT
1. Freestyle midium에서 suspension 293 cell culture
2. Bioreator로 옮겨줌
3. DNA-PEI complex(pTT-EPO+PEI)로 tansfection 수행
4. 4시간 후 293 SFM II medium 첨가
5. 이틀 후 enriched medium에서 fed-batch culture
CSMA/CD=
1장. IEEE 802.3 & Ethernet 관련용어
① IEEE 802 :-IEEE의 LAN과 관련된 표준안이 IEEE 802입니다. IEEE 802에는 CSMA/CD, token bus, token ring등의 대한 표준이 정의되어있습니다. 이것들은 OSI 7 layer중에서 physical layer를 의미하는 것입니다. 한가지 중요한 것이 있습니다. CSMA/CD, token bus, token ring등은 physical layer에
HSV2
-Symptoms of herpes simplex virus infection include watery blisters in the skin or mucou membranes of the mouth, lips or genitals.
-as neurotropic and neuroinvasive viruses, HSV-1 and -2 persist in the body for the life of the carrier by becoming latent and hiding from the immune system in the cell bodies of nerves.
-After the initial or primary infection, some infected people experienc