a sticky end. One of the earlier enzymes to be used, it is popular for historical reasons, but also because digestion leaves a GATC overhang compatible with many other enzymes. Persistent issues with this specific enzyme spontaneously failing to generate cloneable fragments necessitate aliquoting prior to use. Engineered variants of the enzyme have been created in order to avoid star activity.
Reaction은 액상반응이다. Ethylene이 기화되지 않도록 Bubble Point 대비 일정 Margin 이상의 압력에서 반응이 일어나도록 하고 있다. 아래의 Data를 구하시오
Alkylation Bed 1 : 27.42 kg/㎠_g에서 Vapour Fraction=0으로 100% Liquid 상태
같은 방법으로 Alkylation Bed 2에 대해 실행
Distillate Rate 를 240 tonne/h로 지정
1.실험 목적
- 분리한 plasmid DNA를 제한효소로 처리하고, 전기 영동법과 PCR을 이용하여
DNA를 분리하고 확인하는 기술을 습득한다. 제한효소의 종류에 따른 절단 방식을
알아본다.
2. 실험 원리
1) 제한 효소 (Restriction enzyme)
(1) 제한 효소의 정의
* 제
instrument composition
-Electric furnace
Temperature range:
25°C~1500°C
Heat speed :
Maximum 200°C/min
*Separate Electric furnace and other system like Electronic scale
-Because Electric furnace temperature is very high
so we need to protect other system.
instrument composition
-Electric furnace
Temperature range:
25°C~1500°C
Heat speed :
Maximum 200°C/min
*N
Sulfur Dioxide(SO2)
The release of SO2 to the atmosphere is the primary cause of acid rain in the world.
Fossil fuel combustion at electric power-generating plants accounts for about70% of
the emissions.
Coal, Oil, and all other fossil fuels naturally
contain some sulfur.
Volcanic eruptions are another more localized natural source of SO2
SO2 + OH. HSO3.
HS